Katie Boulter's run at this year's Wimbledon singles championships is over after she was beaten 6-7(7), 6-2, 6-1 by Argentina's Solana Sierra.
Boulter had been looking to build on her impressive first-round victory over ninth-seed Paula Badosa, but she wasn't the only player to cause an upset on the opening day.
World No. 101 Sierra, who failed to come through qualifying before winning a lucky loser, is playing at the All England Club for the first time.
Sierra's good fortune has continued this week -- she also fought for her place into the second-round after taking down Australia's Olivia Gadecki -- and the 21-year-old caused another such upset on Wednesday.
Boulter edged the opening set with a narrow 9-7 victory in a tie break. However, Sierra would not be deterred and broke the home favoruite three times to level the match.
From there, it was one-way traffic in favour of the Argentine. She broke Boulter twice more to open a 4-0 lead in the third-set decider before closing out the match.
She will face Spain's Cristina Bucsa in the third round.
"It feels amazing, I knew it was a really tough match, Katie is an amazing player," Sierra said.
"I tried to focus on myself and enjoy the moment because it is a dream to play here in Wimbledon, on this court and with this crowd.
"From the beginning, I believed in myself that I could win this match, so I'm super happy."
British hopes in the women's singles draw have not ended, though. Sonay Kartal enjoyed a 6-2, 6-2 win over Bulgaria's Viktoriya Tomova earlier on Wednesday, while Emma Raducanu put together an impressive performance to beat Marketa Vondrousova.
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